The Rôle of Bacteria in Autolyzing Tissue.
نویسندگان
چکیده
Autolysis by definition designates a process of self-digestion and specifically refers to the dissolution of tissues by enzymes present in the tissue cells, without the aid of micro-organisms. Wells (1925) has given an extensive review of the literature on this subject, the details of which are not within the scope of this paper. Autolysis has been widely studied in animal fluids and tissues, the general thought being that such tissues when aseptically removed are sterile. Most investigators, however, who recognize the difficulty of maintaining asepsis in autolysis experiments, have added small amounts of toluene, chloroform, or other preservative to the autolyzing digest; the idea being that the preservative, while not absolutely inhibitory to enzyme action would nevertheless prevent the growth of bacteria. Bradley and his associates (1915) in an extensive review of autolysis covering many years, record no attempt to determine the possible presence of bacteria in their preparations. Much of their work was done with animal liver incubated with chloroform water or toluene water as a preservative. Recently Herron and McEllroy (1933) report that autolysis of beef liver in N/50 HCl to which a small amount of chloroform was added as a preservative, increased the potency of the liver material in the treatment of pernicious anemia. Mapson (1932) has also reported that the autolysis of beef liver at a pH of 4.5 to 5 increased the potency
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عنوان ژورنال:
- Journal of bacteriology
دوره 31 2 شماره
صفحات -
تاریخ انتشار 1936